首页 | 本学科首页   官方微博 | 高级检索  
     

矛尾复鰕虎鱼病原哈氏弧菌的鉴定及特异性检测方法的建立
引用本文:张晓君,毕可然,阎斌伦,秦蕾,梁立国. 矛尾复鰕虎鱼病原哈氏弧菌的鉴定及特异性检测方法的建立[J]. 水产科学, 2011, 30(12): 758-763
作者姓名:张晓君  毕可然  阎斌伦  秦蕾  梁立国
作者单位:淮海工学院海洋学院,江苏省海水养殖动物病害重点实验室,江苏连云港222005
基金项目:江苏省水产三项工程项目,江苏省六大人才高峰基金资助项目,江苏省自然科学基金资助项目
摘    要:取体表出血、肌肉溃疡的虾蟹养殖塘混养大量死亡的矛尾复鰕虎鱼的深层溃烂组织及肝脏进行细菌分离,2种被检组织中均分离到2种优势生长菌落;人工感染试验表明,其中的一株分离菌(S090801)浓度为106~108 cfu/ml可引起矛尾复鰕虎鱼全部死亡,该菌的形态特征、生理生化特性及基于16SrRNA和gyrB 2种基因的系统发育学分析确定为弧菌属的哈氏弧菌。同时基于gyrB基因序列设计1对特异性引物,建立了一种哈氏弧菌快速、敏感的PCR检测方法,扩增目的片段大小为363bp,该方法检测哈氏弧菌模板DNA最低质量浓度为0.046875ng/μl,可用于哈氏弧菌引起的水产动物疾病的快速诊断及分子流行病学的调查研究。

关 键 词:矛尾复鰕虎鱼  哈氏弧菌  16S rRNA基因  gyrB基因  PCR

Identification and PCR Detection of Pathogenic Vibrio harveyi Isolated from Gobby Synechogobius hasta
ZHANG Xiao-jun,BI Ke-ran,YAN Bin-lun,QIN Lei,LIANG Li-guo. Identification and PCR Detection of Pathogenic Vibrio harveyi Isolated from Gobby Synechogobius hasta[J]. Fisheries Science, 2011, 30(12): 758-763
Authors:ZHANG Xiao-jun  BI Ke-ran  YAN Bin-lun  QIN Lei  LIANG Li-guo
Affiliation:( College of Ocean,Jiangsu Key Laboratory of Animal Diseases in Mariculture,Huaihai Institute of Technology,Lianyungang 222005,China)
Abstract:The outbreaks of mass mortality of pond-cultured gobby Synechogobius hasta occurred in some ponds in Ganyu,Jiangsu province from August to September in 2009.Two dominant bacteria were isolated from liver and ulcer tissues in the gobby with haemorrhages at the body surface,ulcer of muscle,and all inoculated to the fish,leading to died,post-injection with 0.1 ml live cells(106~108cfu/ml,S090801) per fish.The morphological,physiological and biochemical characteristics,and phylogeney analysis based the 16S rRNA and gyrB genes showed that the pathogen in the diseased gobby was considered to be a bacterium Vibrio harveyi.In addition,based on the gyrB gene sequences,a pair of specific primers was designed,and then a rapid and accurate polymerase chain reaction(PCR) detecting V.harveyi were established.The PCR amplified 363 bp gene fragment from chromosomal DNA of V.harveyi,and detect purified chromosomal DNA at a level as low as 0.046875 ng/μl.The PCR protocol is suggested to be used in the specific and rapid diagnose and epidemiological investigation of the disease caused by V.harveyi.
Keywords:Synechogobius hasta  Vibrio harveyi  16S rRNA gene  gyrB gene  PCR
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号