暗孢节菱孢菌非蛋白类毒素的基本性质研究

用改进的Fries培养基在最佳条件下培养出的暗孢节菱孢菌Arthrinium phaeospermum(corda)M.B.Ellis]滤液,采用硫酸铵沉淀制备蛋白类、非蛋白类粗毒素。经致病性检测,前者生物活性低于后者小分子毒素成分。基本性质研究显示非蛋白粗毒素有一定专化性,但专化性不强,介于专化性与非专化性毒素之间;具有较强的热和紫外线稳定性、对pH敏感,易受碱性物质影响;9种不同极性物质对毒素的生物活性有不同影响,该毒素可能为极性较大的物质。

Basic Properties of the Nonprotein Toxin from Arthrinium phaeospermum

The crude protein toxin and nonprotein toxin were prepared by the ammonium sulphate precipitation from the filtrate of A.phaeospermum cultured in the modified Fries culture medium under the optimum condition.The pathogenicity test showed the former had lower toxic activity than the latter with small molecule.Basic properties indicated that the nonprotein toxin had host-specificity to a certain degree,but not absolutely,and was situated between HST and NHST.Meanwhile,the nonprotein toxin had strong thermal and ultraviolet stabilities,was sensitive to pH,and easily affected by the alkalinity substance.Nine polar substances had different effects on the activity of toxin,which might be polar compound.