| 一种新型血栓溶酶的分离提纯及等电点测定 |
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| 引用本文： | 杜冰,胡新宇,姚汝华. 一种新型血栓溶酶的分离提纯及等电点测定[J]. 沈阳农业大学学报, 2000, 31(4): 350-352 |
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| 作者姓名： | 杜冰 胡新宇 姚汝华 |
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| 作者单位： | 华南理工大学食品学院，广东广州510640 |
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| 摘 要： | 中国根霉12（Rizopus chinesis 12）菌株产生的血栓溶酶存在于发酵液中，首先经过饱和度为60%的硫酸铵盐析处理，再经半透膜透析去离子浓缩后，通过CM-C和Sephadex-G100凝胶层析，浓缩含有活性组分的层析洗脱兴，得到血栓溶酶组制品。经等电聚焦法证明为一纯组分，而且得到它的等电点为8.2。
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| 关 键 词： | 中国根霉 血栓溶酶 分离提纯 等电点 |
| 文章编号： | 1000-1700(2000)04-0350-03 |
| 修稿时间： | 2000-01-05 |
Isolation and Purification of a New plasmin and IDeterminafion of Its Isoelectric Point |
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| DU Bing,HU Xin-yu,YAO Ru-hua. Isolation and Purification of a New plasmin and IDeterminafion of Its Isoelectric Point[J]. Journal of Shenyang Aricultural University, 2000, 31(4): 350-352 |
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| Authors: | DU Bing HU Xin-yu YAO Ru-hua |
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| Abstract: | The Plasmin produced from Rhizopus chinesis 12 exists in fermentation solution. The solution was treated by salting-out(60% saturated ammonium sulfate),then deionized and enriched by dialysis,filtrated by CM-C sephadex-G100 gel. The active substance was condensed. At last the raw Plasmin was obtained. The analysis result of electrofocusing ampholine electrophoresis proved the Plasmin was a pure substance. The isoelectric point was 8.2. |
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| Keywords: | Rhizopus chinesis 12 Plasmin isolation and purification isoelectric point |
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