| 基于SNP标记的黄瓜杂交种纯度鉴定方法 |
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| 引用本文： | 兰青阔,张桂华,王永,赵新,朱珠,崔兴华,郭永泽,程奕. 基于SNP标记的黄瓜杂交种纯度鉴定方法[J]. 中国蔬菜, 2012, 1(6): 58-63 |
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| 作者姓名： | 兰青阔 张桂华 王永 赵新 朱珠 崔兴华 郭永泽 程奕 |
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| 作者单位： | 1.天津市农业科学院中心实验室，天津300381,2天津科润农业科技股份有限公司黄瓜研究所，天津300192 |
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| 基金项目： | 天津市应用基础及前沿技术研究计划项目(10JCYBJC09300);天津市科技支撑计划项目(10ZCGYNC00400) |
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| 摘 要： | 根据国际葫芦科基因组数据库CuGenDB中序列信息，应用高分辨率熔解曲线技术筛选出用于黄瓜杂交种纯度鉴定的SNP位点CLA6（A/G），该位点在33个市售黄瓜品种中的多态信息量为0.401，处于中度多态|结合焦磷酸测序技术，建立基于CLA6位点的SNP-Pyrosequencing黄瓜杂交种纯度鉴定方法。利用该鉴定方法检测黄瓜杂交种优一90粒种子，结果其种子纯度为96.7%，该结果与CLA0位点及SSR鉴定结果相符，表明该鉴定技术适于黄瓜杂交种纯度鉴定，具有用于DUS测试分析的潜力。
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| 关 键 词： | 黄瓜 种子纯度 高分辨率溶解曲线 SNP标记 焦磷酸测序 |
SNP-based Molecular Assay for Cucumber Hybrid Seed Purity Identification by Pyrosequencing |
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| LAN Qing-kuo,ZHANG Gui-hua,WANG Yong,ZHAO Xin,ZHU Zhu,CUI Xing-hua,GUO Yong-ze,CHENG Yi. SNP-based Molecular Assay for Cucumber Hybrid Seed Purity Identification by Pyrosequencing[J]. China Vegetables, 2012, 1(6): 58-63 |
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| Authors: | LAN Qing-kuo ZHANG Gui-hua WANG Yong ZHAO Xin ZHU Zhu CUI Xing-hua GUO Yong-ze CHENG Yi |
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| Affiliation: | 1.Central Lab of Tianjin Academy of Agricultural Sciences，Tianjin 300381，China|2Cucumber Research Institute of Tianjin Kernel Agricultural Science and Technology Co.，Ltd.，Tianjin 300192，China |
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| Abstract: | For the purpose of testing cucumber(Cucumis sativus L.)hybrid purity,a SNP site was screened by high resolution melting(HRM) from 33 commercial cucumber cultivars,whose polymorphism information(PIC)were 0.401.We established the purity test method based the SNP,which was genotyped by pyrosequencing.The method was applied in purity test of’Youyi’cucumber cultivar.The result indicated that 90 were pure hybrid seeds,and the purity was 96.7%,which is coincide with the results of CLA0 and SSR.Overall,the method is fit for purity test of cucumber hybrid and the SNP-pyrosequencing test model is suitable for distinctness,uniformity and stability(DUS)analysis. |
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| Keywords: | Cucumber Seed purity High resolution melting(HRM) Single nucleotide polymorphism(SNP) Pyrosequencing technology |
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