Monohaploid plants from anthers of a dihaploid genotype of Solanum tuberosum L. |
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Authors: | Foroughi-Wehr B Wilson H M Mix G Gaul H |
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Institution: | (1) Gesellschaft für Strahlen- und Umweltforschung, Grünbach 1, D-8059 München, W. Germany |
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Abstract: | Summary Monohaploid Solanum tuberosum plants were produced from the anthers of a dihaploid genotype. From another dihaploid genotype plants containing 36 chromosomes were obtained. For plantlet production anthers containing pollen at the uninucleate microspore stage were inoculated on a Linsmaier and Skoog-based medium supplemented with 1 mg/1 indole-3-acetic acid and 1 mg/1 benzyl aminopurine.Anthers from donor plants grown either in the summer or in the winter responded similarly on a range of media. Anther response in the form of callus induction and root formation was at least partly dependent on the genotype of the donor dihaploid plant. |
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Keywords: | Solanum tuberosum monohaploids dihaploid anther culture |
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