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| 利用色谱聚焦和离子交换色谱快速分离分析薏苡38ku抗真菌蛋白 | |
| 引用本文： | 刘静,赵奎军,潘映红. 利用色谱聚焦和离子交换色谱快速分离分析薏苡38ku抗真菌蛋白[J]. 东北农业大学学报, 2008, 39(1): 23-28 |
| 作者姓名： | 刘静 赵奎军 潘映红 |
| 作者单位： | 东北农业大学农学院,哈尔滨,150030;中国农业科学院作物科学研究所,国家农作物基因资源与基因改良重大科学工程,北京,100081;东北农业大学农学院,哈尔滨,150030;中国农业科学院作物科学研究所,国家农作物基因资源与基因改良重大科学工程,北京,100081 |
| 摘 要： | 联合利用色谱聚焦和离子交换色谱对薏苡种子抗真菌蛋白进行了快速分离分析,首次发现一种新的薏苡抗真菌蛋白。该蛋白经SDS-PAGE检测相对分子质量在38左右,利用胶内酶切和MALDI-TOF-MS完成了肽谱分析,并进行了数据库搜索。 |
| 关 键 词： | 薏苡 抗真菌蛋白 色谱聚焦 |
| 文章编号： | 1005-9369（2008）01-0023-06 |
| 收稿时间： | 2006-10-25 |
| 修稿时间： | 2006-10-25 |
Isolation and analysis of 38 ku anti-fungal protein from Coix lacryma-jobi Linn by chromatofocusing and ion-exchange chromatography |
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| LIU Jing,ZHAO Kuijun,PAN Yinghong. Isolation and analysis of 38 ku anti-fungal protein from Coix lacryma-jobi Linn by chromatofocusing and ion-exchange chromatography[J]. Journal of Northeast Agricultural University, 2008, 39(1): 23-28 | |
| Authors: | LIU Jing ZHAO Kuijun PAN Yinghong |
| Abstract: | Chromatofocusing and ion-exchange chromatography were combined to rapidly isolate and analyze antifungal proteins in Coix lacryma-jobi Linn seed. A new antifungal protein in Coix lacryma-jobi Linn seed was first found. Its relative molecular mass was about 38 by SDS-PAGE determination and its peptide mass finger-print was obtained through in-gel-digestion and MALDI-TOF-MS analysis. The peptide mass fingerprint data obtained then were searched in the public database. |
| Keywords: | Coix lacryma-jobi Linn antifungal protein chromatofocusing |
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