Shift of sensitivity of <Emphasis Type="Italic">Botrytis cinerea</Emphasis> to azoxystrobin in greenhouse vegetables before and after exposure to the fungicide |
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Authors: | Chuan-qing Zhang Ya-hui Liu Lan Ding Guo-nian Zhu |
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Institution: | (1) College of Agriculture and Food Science, Zhejiang Agriculture & Forest University, Lin’an, China;(2) Extension Centre of Agriculture Technology of Lin’an City, Lin’an, Zhejiang Province, China |
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Abstract: | From 2004 to 2006, 213 isolates of Botrytis cinerea never exposed to QO center inhibitors (QOIs) were collected to determine the baseline sensitivity to azoxystrobin. In the absence of salicylhydroxamic acid (SHAM),
the mean EC50 values were 10.49 ± 13.12 and 0.36 ± 0.48 mg l−1 for inhibiting mycelial growth and conidium germination, respectively. In the presence of SHAM, the mean EC50 values were 2.24 ± 1.29 and 0.22 ± 0.11 mg l−1. In 2010, five azoxystrobin-resistant isolates were detected with the resistance frequency of 2.25% in greenhouse tomatoes
after 4 years of continuous exposure. These resistant isolates showed cross-resistance to other QOIs but not to boscalid. In addition, these resistant isolates had comparable growth, sporulation and pathogenicity ability
as sensitive isolates and maintained resistance in plants and the presence of SHAM. The G143A point mutation predicted to
cause a change from glycine to alanine at codon 143 of cyt b gene was found in all resistant isolates. |
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