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Growth hormone-like substance in the rotifer Brachionus plicatilis
Authors:Wenresti G Gallardo  Atsushi Hagiwara  Kenji Hara  Kiyoshi Soyano
Institution:Graduate School of Marine Science and Engineering, Nagasaki University, Bunkyo, Nagasaki 852-8131,;Faculty of Fisheries, Nagasaki University, Bunkyo, Nagasaki 852-8521, Japan
Abstract:ABSTRACT:   A growth hormone (GH)-like substance was extracted from the rotifer Brachionus plicatilis and subsequently purified by gel filtration and ion exchange chromatography. The GH-like substance had a molecular weight of approximately 28 kDa and had cross-reactivity with salmon GH antibody. In vivo bioassay showed a higher intrinsic rate of increase and net reproduction rate of B. plicatilis treated with the GH-like substance.
Keywords:Brachionus plicatilis            extraction  GH-like substance  reproduction  Rotifera
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