甘蓝型油菜5个同质CMS系及其保持系、杂交种的同工酶比较

以甘蓝型油菜5种同质异核不育系及相应保持系、5种恢复系、15种杂交F1为试材,进行酯酶(EST)、过氧化物酶(POD)同工酶分析。结果表明:各不育系与其近等基因系——保持系的EST、POD同工酶酶谱较为相似,而5种不育系间及5对不育系与保持系间的差异无规律可循。聚类结果表明聚在一起的类群均有亲缘关系。同工酶表现为明显的共显性,EST尤为明显,不同材料具有各自特殊条带,杂种F1综合了双亲条带,因而也有与每一亲本不同之处。说明共显性的EST等同工酶可用于分析亲缘关系、建立品种指纹及鉴定杂交种纯度等。

Comparison of Some Isozymes among Five CMS Lines with Same Cytoplasm and Their Maintainers in Brassica napus L.

The zymogram of esterase and peroxidase are examined using PAGE in 5cytoplasm male sterility lines with same cytoplasm,their maintainer,and their hybrid,those are all B.napus L.The results showed that every sterile line had similar isozyme pattern to its maintainer,and that their differences had no rule.These materials and their progenies that had similar pattern were clustered in near groups.These isozymes were codominant,especialy in EST.Every accession has specific bands.Nearly every hybrid had all its parent''s bands,so it was different from its parent.Such codominant isozymes as EST and POD can be used to determine the parentage of different accessions,to constuct their fingerprint,and to examine the purity of the hybrid.