Cell and nuclear degradation in root meristems following exposure of potatoes (Solanum tuberosum L.) to salinity |
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Authors: | K. V. A. Richardson A. C. Wetten P. D. S. Caligari |
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Affiliation: | (1) Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, PO Box 221, RG6 6AS Reading, UK;(2) Present address: Instituto de Biologia Vegetal y Biotechnologia, Universidad de Talca, 685 2 Norte, Talca Chile |
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Abstract: | Summary In many plant species, it has been demonstrated at the whole plant level that supplemental Ca2+ alleviates the effects of salinity stress. These effects have been attributed to physiological processes, but there are no reports of the effects of supplemental Ca2+ on preventing nuclear damage to the root meristematic cells following exposure to NaCl salinity. Two in vitro cultured potato (Solanum tuberosum L.) cultivars, analysed by fluorescent microscopy and flow cytometry, showed a similar pattern of salinity-induced changes to the nuclei of root meristematic cells. Damage occurring after only a few hours was followed by nuclear degradation at 24 h. Flow cytometry histograms showed a reduction in G1 and G2 nuclei and an increase in degraded nuclei, in NaCl-stressed roots. Salinity-induced nuclear degradation was alleviated by the addition of CaCl2. |
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Keywords: | flow cytometry calcium alleviation fluorescent microscopy NaCl |
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