农杆菌介导的植物遗传转化影响因素研究进展 |
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引用本文: | 熊换英,钟伟光,张寿文. 农杆菌介导的植物遗传转化影响因素研究进展[J]. 安徽农业科学, 2012, 40(17): 9214-9217,9287 |
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作者姓名: | 熊换英 钟伟光 张寿文 |
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作者单位: | 江西中医学院,江西南昌330004;中国医学科学院药用植物研究所,北京100193;江西中医学院,江西南昌,330004 |
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摘 要: | 农杆菌介导的高效遗传转化体系建立的关键是优化T-DNA导入的各因素,优化外植体、菌株、侵染及共培养条件等因素。文中详细阐述了农杆菌介导植物遗传转化的主要影响因素,为实践操作提供了理论依据。
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关 键 词: | 农杆菌 遗传转化 影响因素 进展 |
Research Progress in the Influencing Factors of Agrobacterium-mediated Genetic Transformation of Plants |
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Affiliation: | XIONG Huan-ying et al(Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi 330004) |
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Abstract: | The key to establish an agrobacterium-mediated high efficient genetic transformation system is to optimize the factors of transforming T-DNA into plant,including the factors of explant,strains,infection,co-culture conditions and so on.This paper stated the role factors in agrobacterium-mediated plant genetic transformation in details,in order to provide theoretical basis for the practical operation. |
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Keywords: | Agrobacterium Genetic transformation Influencing factors Progress |
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