| 重组麻疯树核糖体失活蛋白表达及其体外抗肿瘤活性研究 |
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| 引用本文： | 徐单单,张波,黄亚东,张齐好,苏志坚,许华,郑青. 重组麻疯树核糖体失活蛋白表达及其体外抗肿瘤活性研究[J]. 安徽农业科学, 2012, 0(12): 7079-7082 |
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| 作者姓名： | 徐单单 张波 黄亚东 张齐好 苏志坚 许华 郑青 |
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| 作者单位： | 1. 暨南大学药学院,广东广州,510632
2. 暨南大学医药生物技术研究开发中心,广东广州,510632 |
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| 摘 要： | [目的]研究重组麻疯树核糖体失活蛋白的表达及其体外抗肿瘤活性。[方法]从麻疯树叶中克隆了curcin基因,在此基础上构建pET-22b/curcin重组表达载体,在大肠杆菌中成功表达29 KD的重组蛋白。[结果]经0.5 mM的IPTG在30℃诱导6 h,重组蛋白表达量约占菌体总蛋白的20%,重组curcin以包涵体的形式表达。经溶解、复性、纯化,获得了纯度为95.6%的重组curcin蛋白。[结论]体外研究表明,重组蛋白对A549、HepG2的肿瘤细胞有明显的增殖抑制作用,其IC50分别为92.246和691.42μg/ml,但是对HELA细胞增殖没有明显抑制作用。
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| 关 键 词： | 麻疯树(Jatropha curcas L.) curcin 克隆 表达 纯化 抗肿瘤 |
Expression of Recombinant Curcin in Jatropha curcas L.and Its Antitumour Activity in vitro |
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| Affiliation: | XU Dan-dan et al(College of Pharmacy,Jinan University,Guangzhou,Guangdong 510632) |
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| Abstract: | [Objective] To study the expression of recombinant curcin in Jatropha curcas L.and its antitumour activity in vitro.[Method] Curcin was cloned from the leaves of J.curcas,and the recombinant vector pET-22b was established.The recombinant protein(29KD) was expressed in E.coli.[Result] Induced by 0.5 mM IPTG for 6 h at 30 ℃,expressing quantity of recombinant protein was about 20% of the total protein.Recombinant curcin was expressed in the form of inclusion body.After dissolution,renaturation and purification,purity of recombinant curcin reached 95.6%.[Conclusion] In vitro research showed that recombinant protein had significant proliferation inhibition effects on the A549 and HepG2 tumor cells.And their IC50 were 92.246 and 691.42 μg/ml,respectively.But the recombinant protein had no significant inhibitory effects on Hela. |
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| Keywords: | Jatropha curcas L. Curcin Clone Expression Purify Anti-tumor |
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