浙贝母鳞茎酚类物质含量及相关酶活性与休眠解除的关系

[目的]探讨浙贝母鳞茎中酚类物质及相关酶活性与休眠解除的关系。[方法]将浙贝母鳞茎分别在4和10℃条件下保湿贮藏至75 d,贮藏过程中定期取样测定鳞茎各部位酚类物质含量、苯丙氨酸解氨酶(PAL)活性和多酚氧化酶(PPO)活性,同时比较了发芽鳞茎与休眠鳞茎中酚类物质含量和PAL与PPO活性的差异。[结果]低温解除休眠过程中,鳞茎及芽内酚类物质含量均呈降低趋势;10℃处理30～45 d和4℃处理45～60 d是酚类物质减少、解除休眠的关键时期;浙贝母各部位酚类物质含量与其PPO活性均呈显著负相关;顶芽中酚类物质含量与PAL活性呈显著正相关;鳞片中酚类物质含量与PAL活性没有明显的相关性。[结论]酚类物质与浙贝母鳞茎休眠有一定相关性,酚类物质含量下降是低温处理解除浙贝母鳞茎休眠的生理机制之一。

Changes of Phenolic Compounds Content and Activity of Enzymes Related to Phenolic Compounds in Fritillaria thunbergii Miq.Bulbs Stored at Different Cold Temperatures for Breaking Dormancy

[Objective] To analyze changes of phenolic compounds content and activity of enzymes related to phenolic compounds in Fritillaria thunbergii Miq.bulbs stored at different cold temperatures for breaking dormancy.[Method] Bulbs of F.thunbergii were stored in moist sawdust up to 75 d at 4 or 10 ℃.The samples were taken periodically,and were used to determine phenolic compounds content,PAL activity,PPO activity in different parts of bulb.Differences in phenolic compounds content,PAL activity and PPO activity between dormant and dormancy-broken bulbs were analyzed.[Result] The results showed that a downward trend of phenolic compounds content in bulb was observed during the breaking of dormancy;10 ℃ for 30-45 d and 4 ℃ for 45-60 d were the crucial period of dormancy release and the phenolic compounds decrease;phenolic compounds content and PPO activity were negatively correlated;phenolic compounds content and PAL activity in buds were significantly positive correlation;phenolic compounds content and PAL activity in bulbs had no significant correlation.[Conclusion] There were certain correlations in phenolic compounds of F.thunbergii and its dormancy.Phenolic compounds content drop in F.thunbergii was the physiological mechanism of dormancy release by low temperature treatment.