犬瘟热病毒的分离与鉴定

目的]分离与鉴定犬瘟热病毒(CDV)的石河子流行株。方法]对临床症状疑似犬瘟热和血清学(ELISA)检测为阳性的自然发病犬,取其淋巴结为病料,接种于非洲绿猴肾细胞系(Vero),进行病毒的分离;用RT-PCR检测感染CDV分离株特异性核酸。结果]病料接种Vero细胞产生明显的细胞病变(CPE),用RT-PCR技术可扩增出CDV特异性核酸,扩增出的基因片段与预期设计的长度相同,所扩增的CDV分离株H基因片段与CDV C54标准强毒株核苷酸同源性为98.4%。结论]该研究成功分离并鉴定了CDV石河子流行株,为犬瘟热(CD)的确诊提供了依据。

Isolation and Identification of Canine Distemper Virus

Objective] To isolate and identify the epidemic strains of Canine Distemper Virus(CDV) in Shihezi.Method] The lymph nodes of the nature morbidity dog with suspected clinical symptoms of canine distemper and positive results for ELISA assay were sampled tobe inoculated into the African green monkey kidney cell line(Vero) to isolate canine distemper virus.RT-PCR was used to detect CDV virus nucleic acid to identify the infected virus in cells.Result] The pathological material vaccinated into the Vero cell produced obvious cell pathological change(CPE),with the RT-PCR technology,the CDV specific nucleic acid was amplified,whose length was the same as the predicted,the nucleotide homology between the H gene fragment of CDV isolates and the C54 standard virulent strains of CDV was 98.4%.Conclusion] The CDV was successfully isolated and identified to be an epidemic strains of Shihezi,providing references for the diagnosis of CD.