盐胁迫下外生菌根菌Paxillus抗氧化酶活性的变化

目的]通过测定2种菌株的抗氧化酶活性来研究其抗盐机理。方法]以外生菌根真菌Paxillus involutus的2种菌株MAJ和NAU作为材料,研究在不同NaCl浓度(0、100、200、500 mmol/L)处理条件下抗氧化酶活性的变化。结果]100 mmol/L NaCl可以促进菌株MAJ和NAU的增长及其生物量的增加;短期盐胁迫(1 d)诱导了菌株MAJ中SOD、CAT和POD活性的升高,但随着盐处理时间的延长,这些酶活性在100和200 mmol/L盐胁迫下均有所降低;而菌株NAU在长期盐胁迫(9 d)下能更好地维持各抗氧化酶活性,尤其是APX、GPX和POD。结论]盐处理的菌株抗氧化酶活性明显高于对照菌株。

Changes of Anti-oxidative Enzymes Activity of Paxillus involutus under Salt Stress

Objective] Salt resistance mechanism of two strains of antioxidant enzymes was studied through the determination of their activity.Method] The effects of different concentration of NaCl on anti-oxidative enzymes activity(SOD,CAT,APX,POD and GPX) of Paxillus involutus(strains MAJ and NAU) were investigated.Result] The growth and biomass of the two strains MAJ and NAU were both improved under 100 mmol/L NaCl treatment.Activities of anti-oxidative enzymes were increased in strain MAJ after a short-term salinity(1 d).However,the activity of anti-oxidative enzymes declined with the increase of NaCl concentrations and the duration of salt treatment.Under a long-term salinity(9 d),strain NAU remained the activities of anti-oxidative enzymes,especially APX,GPX and POD.Conclusion] Anti-oxidantive enzymes activity of salt treatment were higher than those of control evidently.