致病疫霉拮抗芽孢杆菌的筛选及脂肽类物质分离的研究

目的]筛选致病疫霉Phytophthora infestans(Mont.)de Bary]拮抗芽孢杆菌并对其脂肽类物质进行分析。方法]采用平板分离培养法从狗尾草Setaria viridis(L.)Beauv]根际土壤中分离产芽孢细菌,采用对峙培养法对其进行初筛和复筛,并对其中杀菌效果较好的Y-3菌株的脂肽类物质进行了HPLC分析。结果]初筛有9株细菌对P.infestans具有一定的拮抗作用,复筛得到Y-1和Y-3菌株对P.infestans的生长具有明显的抑制作用,抑菌率达80%以上;Y-3菌株产生的脂肽粗提物对P.infestans具有较好的拮抗作用,抑制率达90%以上,HPLC分析显示其脂肽粗提物主要包括8种组分。结论]Y-3菌株产生的脂肽类物质抑菌效果显著,在生物防治马铃薯晚疫病方面具有潜在的应用价值。

Screening of Antagonistic Bacillus spp.Strains against Phytophthora infestans ( Mont.) de Bary and Isolation of Lipopeptide Substances

Objective] The paper was to screen the antagonistic Bacillus spp.strains against Phytophthora infestans(Mont.) de Bary and analyze its lipopeptide substances.Method] Using flat isolation and culture method,the bacteria that could produce Bacillus were isolated from rhizosphere soil,and carried out primary and secondary screening by confrontation culture.The lipopeptide substances of Y-3 strain with better antifungal effect were carried out HPLC analysis.Result] Nine strains of bacteria had certain antagonistic effects against P.infestans in primary screening,Y-1 and Y-3 strains had significant inhibition effect against the growth of P.infestans in secondary screening,with inhibition rate higher than 80%;the crude extract of lipopeptide produced by Y-3 strain had better antagonistic effect against P.infestans,with inhibition rate higher than 90%.HPLC analysis showed that the crude extract of lipopeptide mainly contained 8 components.Conclusion] The lipopeptide substances produced by Y-3 strain had significant inhibition effect,which had potential application value for biological control of potato late blight.