1/4野血犊牦牛心肌和骨骼肌线粒体抗氧化酶的活性研究

[目的]探讨犊牦牛线粒体氧自由基代谢水平以及能量代谢的生物学机制。[方法]对青海省大通种牛场6月龄1/4野血犊牦牛心肌和骨骼肌线粒体总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和乳酸脱氢酶(LDH)活性以及丙二醛(MDA)、一氧化氮(NO)含量进行了测定。[结果]野血犊牦牛心肌线粒体中T-AOC、GSH-Px、SOD活性和NO含量均高于骨骼肌,但差异均不显著(P0.05)。心肌线粒体中MDA含量显著高于骨骼肌(P0.05)。[结论]该研究从发育学角度为探讨1/4野血牦牛高原低氧适应性提供理论依据。

Study on the Anti-oxidative Enzyme Activities in Myocardial and Skeletal Muscle Mitochondrion in Yak Calf with 1/4 Wild Blood

[Objective]The research aimed to discuss the molecular biological mechanism of oxygen free radicals metabolism levels and energy metabolism of mitochondria in yak calf.[Method]The activities of total antioxidant capacity(T-AOC),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),lactate dehydrogenase(LDH)and the content of malondialdehyde(MDA),nitric oxide(NO)of myocardial and skeletal muscle mitochondrion of six-month-old yak calf with 1/4 wild blood in Datong cattle breeding farm of Qinghai Province were determined.[Result]The activities of T-AOC,GSH-Px,SOD and NO content in myocardial mitochondrion of yak calf with wild blood were all higher than that of skeletal muscle,but the difference was not significant(P>0.05).The content of MDA in myocardial mitochondrion was significantly higher than that of skeletal muscle(P<0.05).[Conclusion]The research provided theoretical basis for discussing the adaptability to high altitude hypoxia of yak with 1/4 wild blood.