SSR方法标记桃果肉近核色素

目的]利用SSR分子标记法标记桃(Prunus persica(L.)Batsch)果肉近核色素。方法]以"重阳红"与"金保"2个桃品种为亲本构建正交F1群体,选取其中138株后代作为标记群体,采用分离群体分组分析(bulked segregant analysis,BSA)法,将果肉近核色素分为"有"和"无"2个基因池,应用SSR分子标记技术寻找与桃果肉近核色素性状基因连锁的分子标记。结果]通过对256对引物的筛选,获得了3对与控制桃果肉近核色素性状基因连锁的分子标记,即UDP96-003、ch04g09和UDP97-402,同时计算得到这3个标记与桃果肉近核色素性状基因的遗传距离分别为16.7、10.1和17.0 cM。结论]该研究为进一步筛选遗传距离更近的共显性分子标记奠定了基础。

Study on SSR Markers Linked to Flesh Color around the Stone of Prunus persica ( L.) Batsch

Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica(L.) Batsch.Method] P.persica(L.) Batsch varieties Chongyanghong and Jinbao were used as parents to construct F1 orthogonal group.138 F1 individuals were selected as experimental materials for construction of color around the stone gene pool(B1) and non-color around the stone gene pool(B2) by using bulked segregant analysis(BSA) method,molecular markers linked to the flesh color around the stone of P.persica(L.) Batsch were selected with SSR molecular marker technology.Result] After selection with 256 pairs of SSR primers,three pairs of molecular markers linked to the gene controlling flesh color around the stone of P.persica(L.) Batsch were selected(UDP96-003,ch04g09 and UDP97-402).In addition,genetic distances of the three molecular markers with the gene controlling flesh color around the stone of P.persica(L.) Batsch were calculated,which were 16.7,10.1 and 17.0 cM,respectively.Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.