金钗石斛的离体快繁体系研究 |
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引用本文: | 罗林会,王勤.金钗石斛的离体快繁体系研究[J].安徽农业科学,2012,40(15):8476+8479. |
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作者姓名: | 罗林会 王勤 |
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作者单位: | 遵义职业技术学院,贵州遵义,563006;遵义职业技术学院,贵州遵义,563006 |
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摘 要: | 目的]研究金钗石斛的离体快繁体系。方法]以金钗石斛的种子为材料,采用组培法进行离体培养。结果]适宜于金钗石斛圆球茎诱导增殖及芽分化的培养基为:MS+1 mg/L BA+0.02 mg/L NAA+浓度2%马铃薯+浓度3%白糖,增殖系数为7~10。生根培养基为1/2MS+0.2 mg/L BA+0.2 mg/L NAA+0.2 mg/L GA+浓度2%香蕉+浓度2%马铃薯+浓度2%白糖;生根率达98%以上,移栽成活率在90%左右。结论]该方法对金钗石斛的离体快繁体系进行了研究,为组培繁殖金钗石斛种苗提供了参考。
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关 键 词: | 金钗石斛(Dendrobium nobile Lindl.) 离体快繁 培养基 |
Study on the in vitro Rapid Propagation System of Dendrobium nobile Lindl |
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Institution: | LUO Lin-hui et al(Zunyi Vocational and Technical College,Zunyi,Guizhou 563006) |
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Abstract: | Objective] To study the in vitro rapid propagation system of D.nobile.Method] The seeds of D.nobile were selected for the in vitro rapid propagation by tissue culture.Result] MS + 1 mg/L BA + 0.02 mg/L NAA + 2% potato juice +3 % sucrose was suitable for the protocorm induction and bud differentiation of D.nobile.The inductive rate was 7-10.The most suitable rooting medium was 1/2 MS +0.2 mg/L BA + 0.2 mg/L NAA + 0.2 mg/L GA + 2% banana juice + 2% potato juice +2% sucrose.The rooting rate was above 98%,and survival rate was above 90%.Conclusion] The method studied the in vitro rapid propagation system of D.nobile and provided references for the tissue culture of its seedlings. |
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Keywords: | Dendrobium nobile Lindl In vitro rapid propagation Culture medium |
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