| 长寿沙田柚不同外植体愈伤组织诱导的研究 |
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| 引用本文： | 杨玲,王雪梅. 长寿沙田柚不同外植体愈伤组织诱导的研究[J]. 安徽农业科学, 2012, 40(17): 9196-9199 |
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| 作者姓名： | 杨玲 王雪梅 |
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| 作者单位： | 西南林业大学林木生物技术实验中心,云南昆明,650224;西南林业大学林木生物技术实验中心,云南昆明,650224 |
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| 基金项目： | 云南省优势特色重点建设学科项目，西南林业大学"大型仪器设备共享平台"资金 |
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| 摘 要： | [目的]对长寿沙田柚不同外植体愈伤组织的诱导进行研究。[方法]以长寿沙田柚无菌苗为材料,分别采用其上胚轴、下胚轴和子叶节段为外植体,研究不同外源激素对愈伤组织诱导的影响。[结果]诱导长寿沙田柚愈伤组织的外植体以下胚轴为佳,激素组合以1.0 mg/L 6-BA+1.0 mg/L 2,4-D的效果最好。[结论]为以后长寿沙田柚高效离体再生体系的建立及其遗传转化研究奠定了基础。
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| 关 键 词： | 长寿沙田柚 无菌苗 愈伤组织 |
Study on Callus Induction from Different Explants of Citrus grandis Osbeck.cv.Shatianyou Changshou |
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| Affiliation: | YANG Ling et al(Experiment Center for Forest Biological Technology,Southwest Forestry University,Kunming,Yunnan 650224) |
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| Abstract: | [Objective] The aim was to study the callus induction from different explants of Citrus grandis Osbeck.cv.Shatianyou Changshou.[Method] Using the Citrus grandis Osbeck.cv.Shatianyou Changshou aseptic seedling as material,the effects of different exogenous hormone on the callus induction were studied by using the epicotyl,hypocotyl and cotyledon section as explants.[Result] The appropriate explant of Citrus grandis Osbeck.cv.Shatianyou Changshou for callus inducting was the hypocotyl,the best hormone combination was 1.0 mg/L 6-BA+1.0 mg/L 2,4-D.[Conclusion] It prepared the ground for the foundation of high performance regeneration system of Citrus grandis Osbeck.cv.Shatianyou Changshou and its genetic transformation research. |
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| Keywords: | Citrus grandis Osbeck.cv.Shatianyou Changshou Aseptic seedling Callus |
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