高效液相色谱-紫外光检测法测定食品中罗丹明B的含量

目的]建立食品中罗丹明B的高效液相色谱-紫外光检测法。方法]将样品中的罗丹明B用丙酮、正己烷提取,经过氧化铝固相萃取柱净化浓缩后,利用高效液相色谱紫外可见光检测器检测其含量。结果]罗丹明B在0.005～2.000 mg/kg浓度范围内,峰面积与浓度呈良好线性关系,相关系数为0.999 98;方法的定量检测限为0.005 mg/kg,且平均回收率高。结论]该方法适用于食品中罗丹明B的测定,并具有快速、准确、灵敏度高的优点。

Determination of Rhodamine B in Food by HPLC-UV Method

Objective] The aim was to establish a method for the determination of Rhodamine B in food by HPLC-UV.Method] Rhodamine B was extracted with acetone/hexane from food samples.After concentrated and purified by alumina cartridge,the Rhodamine B content in the food was determined by using high performance liquid chromatography with ultraviolet visible detector.Result] Within the concentration range of 0.005-2.000 mg/kg,the peak area of Rhodamine B presents good linear relation with the concentration with the related coefficient of 0.999 98.The detection limit of the method is 0.005 mg/kg,and with high average recovery rate.Conclusion] It is a fast and accurate method to detect Rhodamine B in food with high sensitivity.