海参多糖的提取与纯化研究

目的]研究海参多糖的提取与纯化工艺。方法]以大连产刺参为研究对象,采用碱提取法对海参多糖进行提取和纯化。结果]海参多糖的最佳提取工艺为:KOH浓度为3%,料液比1∶50,提取时间为4 h,提取温度为60℃。在此工艺条件下,多糖提取率为20.69%,适于提取海参多糖。采用过氧化氢脱色和醋酸钾沉淀法去除蛋白,海参多糖的最终得率为7.96%,纯度为82.39%。结论]该研究为刺参多糖的提取、纯化及其他相关研究提供参考。

Extraction and Purification of Polysaccharides in Sea Cucumber

Objective] The research aimed to study the extraction and purification technologies of polysaccharides in sea cucumber.Method] Using Stichopus japonicus produced in Dalian as research object,polysaccharides in sea cucumber was extracted and purified by alkaline extraction method.Result] The optimal extraction conditions were as follows: KOH concentration of 3%,solid-liquid ratio of 1∶50,extraction time of 4 h,extraction temperature of 60 ℃.Under these technology conditions,the extraction rate of polysaccharides was 20.69%.This process conditions were suitable for extracting polysaccharides from sea cucumber.Using hydrogen peroxide oxidation discoloration and acetate precipitation method,the protein was removed.And the final yield of polysaccharides was 7.96% and rate of purity was 82.39%.Conclusion] The research provided references for extraction and purification of polysaccharides from S.japonicus and the relevant studies.