| 高效液相色谱法检测酸奶与奶酪中纳他霉素残留 |
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| 引用本文： | 吴旭,张雪峰,其其格,常建军,姜瑞清,郭建敏. 高效液相色谱法检测酸奶与奶酪中纳他霉素残留[J]. 中国动物检疫, 2010, 27(11): 38-39 |
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| 作者姓名： | 吴旭 张雪峰 其其格 常建军 姜瑞清 郭建敏 |
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| 作者单位： | 内蒙古呼和浩特市盛乐经济园区蒙牛乳业(集团)有限公司 |
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| 摘 要： | 目的:建立高效液相色谱法测定酸奶和奶酪中纳他霉素残留的快速检测方法。方法:样品经水:冰乙酸(体积比为40:5)溶剂提取,采用Agela C18色谱柱(5μm,4.6×250 mm)色谱柱,甲醇:水:乙酸=60:40:5(体积比)为流动相,302 nm检测波长,流速为1 mL/min,柱温采取30℃。结果:酸奶和奶酪中纳他霉素含量在0.5~100 mg/kg范围时与峰面积呈良好的线性关系(r=0.999 9),加标回收率90.3%~95.6%(n=7),相对标准偏差(RSD)1.01%。结论:该方法准确度高、重现性好的特点,特别是操作简便、快捷,可以作为企业生产监控的有效方法。
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| 关 键 词： | 高效液相色谱法 纳他霉素 酸奶 奶酪 |
Detection of Natamycin in Yoghurt and Cheese by HPLC |
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| Wu Xu,Zhang Xuefeng,Qiqige,Chang Jianjun,Jiang Ruiqing,GuoJianmin. Detection of Natamycin in Yoghurt and Cheese by HPLC[J]. China Animal Health Inspection, 2010, 27(11): 38-39 |
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| Authors: | Wu Xu Zhang Xuefeng Qiqige Chang Jianjun Jiang Ruiqing GuoJianmin |
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| Affiliation: | 1.Inner Mongolia Mengniu Industrial Group Co.,Ltd.,Inner Mongoliao 011500) |
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| Abstract: | A method for detection of natamycin in yoghurt and chees by HPLC is described.The samples were extracted with glacial acetic acid(40:5) and detected by HPLC with Agela C18 column and methanol:water: glacial acetic acid(60:40:5) as flow phase at 1mL/min and the UV detection wavelength was 302 nm.The mean recoveries were 90.3%~95.6%,and RSD was1.01%.The method was simple,accurate,highly specific and reliable for detection of natamycin. |
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| Keywords: | HPLC natamycin yoghurt cheese |
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