| 拟南芥中热胁迫相关microRNA的差异表达 |
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| 引用本文： | 冯静弦,汪启明,胡琪,饶力群.拟南芥中热胁迫相关microRNA的差异表达[J].湖南农业科学,2012(3):10-13. |
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| 作者姓名： | 冯静弦 汪启明 胡琪 饶力群 |
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| 作者单位： | 湖南农业大学生物化学与分子生物学研究室,湖南长沙,410128 |
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| 基金项目： | 湖南省自然科学基金重点项目(08jj3066) |
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| 摘 要： | 对改良CTAB法、改良热酚法和Trizol法3种方法在提取拟南芥叶片的总RNA中的效果进行了比较,并利用半定量RT-PCR技术检测热胁迫相关microRNA(miRNA)的表达情况。结果表明:Trizol法提取能得到高质量的拟南芥叶片总RNA,效果优于改良CTAB法、改良热酚法;筛选的4个拟南芥miRNA的表达受到热胁迫的诱导,在38℃不同时间处理下各个miRNA的表达情况都有差异,随着处理时间的延长,其表达量均出现明显提高的情况。
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| 关 键 词： | 拟南芥 microRNA RNA提取 热胁迫 RT-PCR |
Differential Expression of Heat Stress Related microRNA in Arabidopsis thaliana |
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| FENG Jing-xian
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WANG Qi-ming
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HU Qi
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RAO Li-qun.Differential Expression of Heat Stress Related microRNA in Arabidopsis thaliana[J].Hunan Agricultural Sciences,2012(3):10-13. |
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| Authors: | FENG Jing-xian WANG Qi-ming HU Qi RAO Li-qun |
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| Institution: | (Research Department of Biochemistry and Molecular Biology,HNAU,Changsha 410128,PRC) |
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| Abstract: | To establish the optimal system for extracting total RNA from leaves of Arabidopsis thaliana,the extraction effects of three methods,which including modified CTAB method,modified hot-phenol method and Trizol method,were compared,meanwhile,the expressions of heat stress related miRNAs were detected by using semi-quantitative RT-PCR technology.The results showed that using Trizol method can extract high quality total RNA of Arabidopsis thaliana,and its extracting effect was better than that of modified CTAB method and modified hot-phenol method;the expressions of the four screened miRNAs were induced by heat stress,but the expressions of each miRNA,which were treated at 38℃ for different time,were different,and with the time passed by,the expression level of each miRNA all was enhanced significantly. |
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| Keywords: | Arabidopsis thaliana microRNA RNA extraction heat stress RT-PCR |
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