斑点叉尾鮰对3种致病菌外膜蛋白(OMP)的免疫应答

利用从嗜水气单胞菌(Aeromonas hydrophila)、柱状嗜纤维菌(Cytophaga columnaris)和叉尾鮰爱德华菌(Edwardsiella ictaluri)中提取的外膜蛋白(outer membrane protein,OMP)作为免疫原,注射接种斑点叉尾鮰(Ictalurus punctatus Rafinsque)后,通过测定受免鱼的交叉凝集抗体效价、肾脏和血液中吞噬细胞的吞噬活性和采用A. hydrophila活菌攻毒方法,比较了3种致病菌OMP对斑点叉尾鮰的免疫原性。试验结果表明:从3种致病菌中提取的OMP对斑点叉尾鮰均具有较强的免疫原性,受免鱼的血清中存在对3种致病菌的交叉凝集抗体,与对照鱼相比,肾脏和血液中吞噬细胞对吞噬原的吞噬活性和对A. hydrophila活菌攻毒的相对免疫保护率(relative percent survival,RPS)明显上升。说明在3种供试菌的OMP上不同程度地存在共同保护性抗原。

Immune Response of Channal Catfish to Outer Membrane Protein of Three Pathogenic Bacteria

Channal catfish(Ictalurus punctatus Rafinsque)were immunized with outer membrane protein(OMP) extracted from Aeromonas hydrophila,Cytophaga columnaris and Edwardsiella ictaluri.The immunogenicity of OMP to channal catfish were compared by examing the agglutinating antibody titers,cross-agglutinating antibody titers of serum,the phagocytic activity of phagocytes in the blood and head kidney and relative percent survival(RPS) by challenging with live A.hydrophila.The results showed that the agglutinating antibody titers and cross-agglutinating antibody titers of immunized channel catfish were higher than the control group.The phagocytic activity of phagocytes in the blood and head kidney of channel catfish was significantly boosted with a high level of immune response.The crude LPS of three pathogenic bacteria were thought to have a common protective antigen which cause a cross-immune response to channel catfish.