除草剂阿特拉津对雄性大鼠血清睾酮水平的影响

将Wister雄性大白鼠70只,随机分成5组(3个处理组,阴性、阳性对照组),处理组和阴性对照组分别按每千克体重225.5、111.5、55.625、0 mg腹腔注射阿特拉津,阳性对照组按每千克体重0.5 mg腹腔注射苯甲酸雌二醇,并于注射后30 d、60 d和90 d采样测定大鼠血清中睾酮的含量.高剂量组30 d、60 d和90 d分别为137.63±73.45、762.66±449.84和89.39士47.15;中剂量组30 d、60 d和90 d血清中睾酮的的含量分别为355.44±507.32、303.87士101.26和171.51±122.51;低剂量组分别为363.54士86.70、94.84±121.22和98.30±51.94.低剂量阿特拉津作用组,大鼠血清睾酮水平3次测得数据呈现一定的下降趋势,而中剂量组3次测得数据呈现递降趋势,高剂量组则在60 d采样时出现睾酮分泌高峰,且30 d和90 d时睾酮水平很接近.各剂量组阿特拉津对大鼠血清睾酮的含量水平均有影响,且30 d时存在时效关系.

Effect of Atrazine on the Concentration of Testosterone in Male Rats

The researching effect of herbicide At razine on the concent ration of serum testosterone in maleness rats lays afoundation approaching other environmentestrogenic hormone.70 Wister rats were randomly divided into 5 groups(3 for treatments,negative and positive control),each treatment group and negative control was injected Atrazine in intraperitoneal in the amount of 222.5 mg·kg^-1 ·BW,111.5 mg·kg^-1 ·BW,55.625 mg·kg^-1 ·BW,0 mg·kg^-1 ·BW respectively.Positive control was injected benzestrofol in int raperito neal in the amount of 0.5 mg·kg^-1 ·BW.The concentration of serum testosterone in malerats were assayed at the 30 th,60 th and 90 th day after giving drug.Experimental result showed that high-dose group in serum testosterone in 30th day,60th day and 90th day were 137.63±73.45,762.66±449.84 and 89.39±47.15;middle-do se group in serum testosterone in 30th day,60th day and 90th day were 355.44±507.32,303.87±101.26 and 171.51 ±122.51;low-dose group in serum testosterone in 30th day,60th day and 90th day were 363.54±86.70,94.84±121.22 and 98.30±51.94.The results indicated that herbicide Atrazine effected the concent ration of serum testosterone in malerats and existed time-effect relationship in 30d.