| 拟南芥菜一个组织特异基因的克隆与研究 |
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| 引用本文： | 刘庆法,刘 勤,王中华. 拟南芥菜一个组织特异基因的克隆与研究[J]. 西北农林科技大学学报(自然科学版), 1999, 27(2): 11-16 |
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| 作者姓名： | 刘庆法 刘 勤 王中华 |
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| 作者单位： | 1. 西北农业大学农学系,陕西杨凌,712100;复旦大学遗传研究所,上海,200433
2. 西北农业大学农学系,陕西杨凌,712100 |
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| 摘 要： | 利用启动子陷阱技术，鉴定和分离了一个在拟南芥菜的维管束和花药的绒毡层细胞中高度特异性表达的基因。经鉴定，该基因全长为４．４ｋｂ，编码一种与ＲＮＡｈｅｌｉｃａｓｅＡ高度同源的蛋白。组织化学和原位杂交的证据表明，该基因只在维管束中少数细胞内转录、翻译并在原位发挥作用。
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| 关 键 词： | 拟南芥菜 基因表达 组织特异性 绒毡层 维管束 基因克隆 |
| 修稿时间： | 1998-09-29 |
Cloning and Studies on a Tissue-Specific Gene in Arabidopsis thaliana Identified by Promoter Trapping |
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| Liu Qingf,Liu Qin Wang Zhonghua. Cloning and Studies on a Tissue-Specific Gene in Arabidopsis thaliana Identified by Promoter Trapping[J]. Journal of Northwest A&F University（Natural Science Edition）, 1999, 27(2): 11-16 |
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| Authors: | Liu Qingf Liu Qin Wang Zhonghua |
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| Affiliation: | 1 (1 Department of Agronomy,Northwestern Agricultural University,Yangling,Shaanxi 712100) (2 Institute of Genetics,Fudan University,Shanghai 200433) |
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| Abstract: | By the aid of promoter trapping,a vascular and tapetum specifically expressed gene was identified,characterized and expressionally analyzed.The results showed that the gene was 4.4 kb in length,coding a protein highly homologous to RNA helicase A.Histochemical and tissue in situ hybridization analysis revealed that it was expressed and processed in xylem and tapetum cells,and functions in these cells only. |
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| Keywords: | Arabidopsis thaliana gene expression tissue specific tapetum vascular gene cloning |
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