黄,白金针菇品系酯酶同工酶标记筛选研究初报

采用垂直平板聚丙烯酰胺凝胶电泳,研究不同生长发育期、不同组织对金针菇[Fla~(?)mmulina velutipes(Fr.)Kar.]黄、白品系酯酶同工酶电泳表型的影响,筛选出不受生长发育期及常规培养条件影响的酯酶标记区带.标记区带分基本带和识别带.酯酶同工酶标记区带电泳表型显示出多态型.利用酯酶同工酶标记区带分析黄、白金针菇酶谱差异,显示出黄、白金针菇品系有共同的遗传基础.

A Preliminary Study on the Screening of Esterase Isozyme Marker of Flammulina velutipes Yellow and White Strains

Orthogonal slab polyacrylamide gel electrophoresis (OSPAGE) was used to study the effect of different development stages and different tissues on the esterase isozyme electrophoresis phenotype of F. velutipes yellow and white strains. And marker bands of esterase isozyme which were not effected by different development stages and routine culture conditions were screened out. Esterase isozyme marker bands could be divided into primary esterase isozyme marker band which could be obtained from all tested strains, and distinguishing esterase isozyme marker bands which could only be found in some strains. It was proved that OSPAGE phenotype of esterase isozyme marker bands was polymorphic, and that using esterase isozyme marker bands to analyse the zymogram differentiation of F. velutipes yellow and white strains, the common genetic base was found to exist in the tested yellow and white strains.