| 猪肺炎霉形体细胞膜的制备方法 |
| |
| 引用本文： | 张淑改 聂向庭. 猪肺炎霉形体细胞膜的制备方法[J]. 山西农业大学学报(自然科学版), 1996, 16(4): 380-382 |
| |
| 作者姓名： | 张淑改 聂向庭 |
| |
| 作者单位： | 山西农业大学 |
| |
| 摘 要： | 本文针对常规低渗破膜效率较差这一特点，从膜纯度，膜产量，膜毒力三方面入手进行了三种细胞裂解方法的比较研究。表明：超声法膜蛋白产量和膜ＡＴＰａｓｅ活性分别比低渗膜提高１０．１３％、２９．６％，但膜毒性降低；低渗超声膜相应指标分别比低渗膜提高１６．７９％，２．９８％，而且膜毒性也有所改观，对猪肺炎霉形体是一种比较理想的细胞裂解方法
|
| 关 键 词： | 猪肺炎霉形体 细胞膜 低渗裂解 超声破碎 低渗超声破碎 |
A SUTDY ON THE METHOD OF MEMBRAME PREPARATION OF MYCOPLASMA HYOPNEUMONIA |
| |
| Zhang Shugai et al.. A SUTDY ON THE METHOD OF MEMBRAME PREPARATION OF MYCOPLASMA HYOPNEUMONIA[J]. Journal of Shanxi Agricultural University(Nature Science Edition), 1996, 16(4): 380-382 |
| |
| Authors: | Zhang Shugai et al. |
| |
| Abstract: | the purpose is to solve the problem of inefficiency of routine method of osmotic.Comparaton of mark enzyme activity,membraine yield and ATPase activity of Vltrasonic irradiation increased by 10 13%,29 6%,but the membrane cytotoxicity was decreased.The same factor of Vltrasonic irradiation-osmotic rised by 16 79%,2 98% and membrane cytotoxicity improved.So the third method is the best method of lysis of hyopneumonia mycoplasma. |
| |
| Keywords: | Hyopneumonia mycoplasma Osmotic Vltrasonic irradiation Osomotic-vltrasonic irradiation Membrane |
| 本文献已被 CNKI 维普 等数据库收录！ |
