Mapping of rhizoctonia root rot resistance genes in sugar beet using pathogen response‐related sequences as molecular markers |
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| Authors: | J C Lein C M Sagstetter D Schulte T Thurau M Varrelmann B Saal G Koch D C Borchardt C Jung |
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| Institution: | 1. Plant Breeding Institute, Christian‐Albrechts‐University of Kiel, Olshausenstrasse 40, D‐24098 Kiel;2. Institute of Sugar Beet Research (IfZ), Associated Institute at the University of G?ttingen, Holtenser Landstrasse 77, D‐37079 G?ttingen;3. Institute of Agricultural and Nutritional Sciences, Martin‐Luther‐University Halle‐Wittenberg, Ludwig‐Wucherer‐Str. 2, D‐06108 Halle (Saale);4. Strube‐Dieckmann, A. Dieckmann Heimburg, Kirchhorster Str. 16, D‐31688 Nienst?dt;5. KWS SAAT AG, Grimsehlstrasse 31, 37555 Einbeck, Germany;6. Corresponding author, E‐mail: c.jung@plantbreeding.uni‐kiel.de |
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| Abstract: | Rhizoctonia root and crown rot caused by the fungus Rhizoctonia solani is a serious disease of sugar beet. An F2:3 population from a cross between a resistant and a susceptible parent has been tested for R. solani resistance and a genetic map has been constructed from the corresponding F2 parents. The map encompasses 38 expressed sequence tags (ESTs) with high similarity to genes which are involved in resistance reactions of plants (R‐ESTs) and 25 bacterial artificial chromosomes (BACs) containing nucleotide binding site (NBS)‐motifs typical for disease resistance genes. Three quantitative trait loci (QTL) for R. solani resistance were found on chromosomes 4, 5 and 7 collectively explaining 71% of the total phenotypic variation. A number of R‐ESTs were mapped in close distance to the R. solani resistance QTL. In contrast, the NBS‐BACs mapped to chromosomes 1, 3, 7 and 9 with two major clusters of NBS‐BACs on chromosome 3. No linkage between NBS‐BACs and R. solani resistance QTL was found. The data are discussed with regard to using R‐ESTs and NBS markers for mapping quantitative disease resistances. |
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| Keywords: | Rhizoctonia solani Beta vulgaris QTL resistance gene analogue functional marker BAC |
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