NaCl胁迫对黑果枸杞幼苗生理及生化指标的影响

以黑果枸杞幼苗为试验材料,将幼苗在0、50、100、200 mmol·L^-1 NaCl条件下处理10 d后,分析了主根伸长量、叶片花青素、游离脯氨酸、丙二醛、可溶性蛋白质含量、超氧化物歧化酶(SOD)活性和过氧化物酶(POD)活性及其同工酶等生理生化指标的变化,探究其适应盐生环境可能的机制,以期为合理开发利用黑果枸杞提供参考依据。结果表明:经0、50、100、200 mmol·L^-1NaCl处理后,黑果枸杞幼苗主根生长未受抑制,0～100 mmol·L^-1 NaCl处理时,叶片花青素含量变化不显著,200 mmol·L^-1 NaCl处理后,花青素含量显著升高;0～100 mmol·L^-1 NaCl处理时,幼苗中游离脯氨酸及丙二醛含量变化不显著,200 mmol·L^-1 NaCl处理后以上物质均显著升高;经0～200 mmol·L^-1 NaCl处理,黑果枸杞幼苗中SOD活性及其同工酶酶谱表达稳定,变化不显著;POD活性先升后降,100 mmol·L^-1 NaCl胁迫时活性最强,为1 065.9 U·g^-1·min^-1,且同工酶POD 1、POD 2与黑果枸杞幼苗响应盐胁迫紧密相关。

Effects of NaCl Stress on Physiological and Biochemical Characteristics of Lycium ruthecium Murr. Seedling

Lycium ruthenicum Murr.seedlings were treated with different medium contained 0,50,100 and 200 mmol·L^-1 NaCl for 10 days,root length increasement,contents of anthocyanin in leaves,proline,MDA,soluble protein,activities and isozymes of POD and SOD response to salinity were analyzed.The effect of different concentration medium of NaCl on physiological and biochemical characteristics of Lycium ruthecium Murr.seedling was studied,which to detect mechanism of the plant adapt to salinity condition and provide scientific basis for future utilizing.The results showed that the increased root length was not influenced by 0-200 mmol·L^-1 NaCl stress medium.In200 mmol·L^-1 NaCl condition,anthocyanin in leaves was raised up and reached the highest level.The content of proline and MDA were not changed significantly under 0 to 100 mmol·L^-1 NaCl stress,while all the characteristics elevated in response to 200 mmol·L^-1 NaCl.With the increasing salinity,the activity and expression of SOD isozymes were stabilized.POD avtivity showed a trend of first rising and then falling along with the salt concentration increasing,the maximization activity was1 065.9 U·g^-1·min-1 in 100 mmol·L^-1 NaCl medium.Isozymes of POD 1 and POD 2 were correlated with seedling salt tolerance.