两株生防细菌对苦瓜枯萎病的防治效果及其定殖密度的研究

以"如玉39"苦瓜为试材,利用皿内和盆栽试验,研究了生防细菌K18-5和KH48-5对苦瓜枯萎病菌的抑制活性和苦瓜枯萎病的防治效果,及其在苦瓜根系的定殖能力,以期为苦瓜枯萎病生物防治提供优质的生防资源。结果表明:K18-5和KH48-5均显著抑制枯萎病菌菌丝生长及其分生孢子的萌发;在盆栽试验中,K18-5和KH48-5对苦瓜枯萎病具有显著的防治效果,其防效分别达到75.27%和69.11%。K18-5和KH48-5均具有良好的根际定殖能力,在皿内采用双层滤纸法测定其定殖密度,分别为2.45×104 cfu·cm^-1和4.60×103 cfu·cm^-1;在盆栽试验中,K18-5和KH48-5在苦瓜根部稳定保持1×103 cfu·g-1以上的定殖密度。

Research on Control Effects of Two Biocontrol Agents Against Fusarium Wilt of Bitter Melon and the Root Colonization Ability

‘Ruyu 39’bitter melon was used as material.In vitro and pot experiments were conducted to research on the inhibitory activities and control effect of two kinds of K18-5and KH48-5biocontrol agents against Fusarium oxysporumand their the root colonization abilities.In order to provide quality biological control resources for balsam pear wilt.The results showed that K18-5 and KH48-5 inhibited the mycelial growth and conidiospore germination of Fusarium oxysporum in vitro.In pot experiment,K18-5and KH48-5had significant control potentials against Fusarium wilt and the control efficiencies were 75.27% and 69.11% respectively.In addition,K18-5and KH48-5 had strong colonization abilities in rhizosphere of bitter melon,the colonization densities were 2.45×10^4 cfu·cm^-1 and 4.60×10^3 cfu·cm^-1 respectively with double layer filter paper method,in pot experiment,K18-5and KH48-5could sustain at high population densities in bitter melon root which were more than 1×103 cfu·g^-1.