铜尾矿渗出液胁迫下不同浓度EM菌肥对植物生长及Cu离子富集的影响

以美人蕉(Canna glauca)和萍蓬草(Nuphar pumilum)为试材,采用盆栽法,研究了不同浓度EM菌肥(1 000、2 000、4 000、8 000倍液)对铜尾矿渗出液中植物生长及Cu离子富集的影响,以期为EM菌肥实际应用于修复和治理矿区废弃尾矿渗出液水质及植物筛选提供依据。结果表明:在铜尾矿渗出液胁迫下,EM菌肥处理下美人蕉幼苗叶数、根数均大于对照;高浓度EM菌肥对美人蕉和萍蓬草株高均有抑制作用,而低浓度对2种植物的根长有促进作用。在不同浓度EM菌肥处理下美人蕉幼苗耐性指数均高于对照,萍蓬草则表现出低浓度EM菌肥处理耐性指数大于对照。在4 000倍液浓度下美人蕉地上部Cu离子含量最高,是对照的1.16倍,而地下部Cu离子含量在4 000倍液时最低。EM菌肥一定程度上促进了美人蕉地下部Cu离子向地上部转移。添加不同浓度EM菌肥一定程上均促进了2种植物对Cu离子的富集,对萍蓬草的促进程度高于美人蕉。

Effects of Different Concentrations of the EM Bacterial Manure on Growth and Cu Ion Enrichment of Canna glauca and Nuphar pumilum Cultivated in CuL

Canna glauca and Nuphar pumilum were used as test materials,the method of potted was adopt,the effects of different concentrations of the EM bacterial manure(1 000,2 000,4 000,8 000 times solution)on the growth and Cu ion enrichment of two kinds of plants cultivated in copper tailings exudation were studied,in order to provide basis for the application of EM bacteria manure in the restoration of the copper tailings exudation water and plant screening.The results showed that under the copper tailing effusion stress,the number of leaves and roots of C.glaucaseedlings which were treated with EM bacterial manure at different concentrations were higher than control.High concentration of EM bacterial manure could inhibit the height of both C.glauca and N.pumilum,while low concentration could promote the root length of both plants.The tolerance index of C.glauca seedlings was higher than control at different concentrations of EM,while the tolerance index of N.pumilumseedlings was higher than control at low concentrations of EM.The Cu ion content(4 000 times solution)in the aboveground part of C.glauca was the highest,1.16 times that of the control,the Cu ion content in the underground part was the lowest at EM 4 000 times solution.Thus EM bacterial manure promoted Cu ion transfer in the underground part of C.glaucato the aboveground part to some extent.The experimental results also indicated that the addition of EM bacterial manure with different concentrations promoted the enrichment of Cu ions in both plants,and the effect of the N.pumilum was better than that of C.glauca.