大花红景天谷胱甘肽 S-转移酶基因的分离与鉴定

以西藏大花红景天为试材,利用生物信息学和实时荧光定量PCR技术对谷胱甘肽S-转移酶(GST)基因家族中的11个成员进行分析,以期为红景天属GST家族基因的功能研究提供参考。结果表明:基于蛋白质序列比对可以将11个大花红景天RcGSTs基因分为6个亚组,分别为tau类(RcGSTU1、RcGSTU2、RcGSTU3);phi类(RcGSTF1、RcGSTF2、RcGSTF3、RcGSTF4);lambd类RcGSTL;微粒体类RcGSTM;theta类RcGST1;zeta类RcGSTZ。在线网站分析结果显示仅RcGSTM具有跨膜结构域,并定位于细胞膜,其它RcGSTs均无跨膜结构域并定位于细胞质。组织特异性与胁迫诱导表达模式结果表明,RcGSTs基因具有不同的组织表达特异性;低温胁迫下4个基因表达水平上调,7个基因下调;且脱落酸(ABA)可以诱导RcGSTs基因表达。

Isolation and Investigation of Glutathione S-transferase Genes in Tibet Rhodiola renulata

The glutathione S-transferase(GST) gene family members were analysis by bioinformation and qRT-PCR for Tibet Rhodiola crenulata,in order to lay a foundation for the further studies of function about GST family genes in Rhodiola.The results showed that 11 RcGSTs genes were classified into six subgroups according to protein sequence identity contained tau(RcGSTU1,RcGSTU2,RcGSTU3),phi(RcGSTF1,RcGSTF2,RcGSTF3,RcGSTF4),lambd(RcGSTL),microsomal(RcGSTM),theta(RcGST1),zeta(RcGSTZ).All RcGSTs genes located in cytoplasm and without transmembrane domain expect RcGSTM.The expression patterns for tissue and stresses presented that RcGSTs had different tissue expression specificity.Under cold stress,4 of 11 RcGSTs expression levels were up-regulation and 7 of 11 RcGSTs were down-regulation.For abscisic acid stresses,all RcGSTs were induced the expression levels.