Methods for inoculum production and inoculation of Cistella japonica,the causal agent of resinous stem canker in Chamaecyparis obtusa |
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| Authors: | T. Yamanobe Y. Oribe K. Nishimura |
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| Affiliation: | 1. Kansai Breeding Office, Forest Tree Breeding Center, Uetsukinaka 1043, Shoo, Okayama 709‐4335, Japan;2. E‐mail: yamanobe@affrc.go.jp;3. (for correspondence);4. Tohoku Breeding Office, Forest Tree Breeding Center, Osaki 95, Takizawa, Iwate 020‐0173, Japan;5. Kyushu Regional Office, Japan Forest Tree Breeding Association, Suya 2775‐9, Koshi, Kumamoto 861‐1104, Japan |
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| Abstract: | The ascomycete Cistella japonica was cultured on potato dextrose agar medium (PDA) for inoculation into Chamaecyparis obtusa, enabling the development of an inoculation method suitable for use in a breeding programme aimed at selecting for families of Ch. obtusa resistant to resinous stem canker. Using PDA to generate the inoculum resolved the problems encountered with the previously used mixed medium of rice bran and wheat bran, including unfavourable characteristics, uncertain growth of Ci. japonica mycelia, and a complex culturing operation. The inoculation test induced resin exudation similar to that observed in natural infections, and reproduced clonal differences with regard to damage severity. |
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