| ‘京欣一号’父本西瓜组织培养研究 |
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| 引用本文： | 程永强,李喜焕,王静华,徐金茹,程琳,段会军. ‘京欣一号’父本西瓜组织培养研究[J]. 河北农业大学学报, 2009, 32(2) |
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| 作者姓名： | 程永强 李喜焕 王静华 徐金茹 程琳 段会军 |
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| 作者单位： | 河北农业大学,河北省作物种质资源重点实验室,河北,保定,071001 |
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| 摘 要： | 为建立简单、快速的西瓜诱导不定芽分化的试验体系,以西瓜‘京欣一号’父本为材料诱导无菌苗,接种4~5 d苗龄的子叶和下胚轴进行组织培养,结果表明:采用子叶为外植体诱导的不定芽数量比下胚轴多;以MS培养基为基本培养基,添加BA 1.0 mg/L和IAA 0.5 mg/L时为最佳不定芽诱导培养基,添加0.1 mg/L KT为最适合的不定芽伸长培养基,添加NAA 0.2 mg/L的1/2MS培养基为最佳生根培养基。
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| 关 键 词： | 西瓜 子叶 下胚轴 组织培养 |
Study on tissue culture of watermelon 'Jing Xin No.1' male parent |
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| CHENG Yong-qiang,LI Xi-huan,WANG Jing-hua,XU Jin-ru,CHENG Lin,DUAN Hui-jun. Study on tissue culture of watermelon 'Jing Xin No.1' male parent[J]. Journal of Agricultural University of Hebei, 2009, 32(2) |
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| Authors: | CHENG Yong-qiang LI Xi-huan WANG Jing-hua XU Jin-ru CHENG Lin DUAN Hui-jun |
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| Affiliation: | Key Laboratory of Crop Germplasm Resources of Hebei;Agricultural University of Hebei;Baoding 071001;China |
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| Abstract: | Cotyledons and hypocotyls at 4-day seedlings induced by seeds of 'Jing Xin No.1' male parent were used to study regeneration system.The results showed that: cotyledons produced much more shoots than hypocotyls;MS supplemented with BA 1.0 mg/L and IAA 0.5 mg/L was the best shoot regeneration medium;To shoot growth,the MS supplemented with 0.1 mg/L KT medium was better than others;1/2 MS supplemented with NAA 0.2 mg/L was the best rooting medium. |
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| Keywords: | watermelon cotyledon hypocotyl tissue culture |
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