| 小白菜小孢子胚状体诱导成苗及利用 |
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| 引用本文： | 王超楠,冯辉,姜凤英,刘如娥,周英. 小白菜小孢子胚状体诱导成苗及利用[J]. 中国蔬菜, 2007, 1(8): 18-21 |
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| 作者姓名： | 王超楠 冯辉 姜凤英 刘如娥 周英 |
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| 作者单位： | 沈阳农业大学农学院，110161 |
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| 基金项目： | 国家自然科学基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 以10个小白菜F1为试材进行游离小孢子培养,研究小孢子胚发生及其成苗的主要影响因素,并对获得的双单倍体以及用其配制的杂交组合的园艺学性状进行鉴定。结果表明,品种间小孢子出胚率差异较大;NLN-13培养基中大量元素减半有利于小孢子胚发生,胚诱导率达100%;培养基中添加适量的6-BA和NAA可以提高小孢子出胚率;小孢子胚成苗培养基的适宜琼脂浓度为1.0%,小孢子胚成活率可达85.88%;MS+3%蔗糖+0.8%琼脂是小孢子再生植株继代和壮苗的适宜培养基,MS+3%蔗糖+0.8%琼脂+NAA0.1mg.L-1是小孢子再生植株生根的适宜培养基;双单倍体及其与自交系配成的杂交组合整齐一致。
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| 关 键 词： | 小白菜 小孢子培养 胚状体 双单倍体 |
| 收稿时间： | 2007-01-11; |
| 修稿时间： | 2007-01-11 |
The Establishment and Application of Isolated Microspore Culture Technology System in Brassica campestris L.ssp.chinensis var.communis Tsen et Lee |
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| Wang Chaonan, Feng Hui, Jiang Fengying,et al.. The Establishment and Application of Isolated Microspore Culture Technology System in Brassica campestris L.ssp.chinensis var.communis Tsen et Lee[J]. China Vegetables, 2007, 1(8): 18-21 |
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| Authors: | Wang Chaonan Feng Hui Jiang Fengying et al. |
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| Affiliation: | College of Horticulture; Shenyang Agricultural University; Liaoning 110161 |
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| Abstract: | Isolated microspore culture was carried out with ten F1 hybrids of pakchoi(Brassica campestris L.ssp.chinensis var.communis Tsen et Lee.).The frequency of embryogenesis,embryos germinated seedling,subculture and rootage of regenerated plantlets,uniform identification of horticultural characters from the DH line progenies and hybrid made with DH lines were studied.The results showed that genotypes had remarkable effect on embryoid induction.Using semi-content of macro-elements in NLN-13 medium increased the ... |
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| Keywords: | Pakchoi Microspore culture Embryoid Double haploid |
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