| 反向遗传学技术及其在口蹄疫病毒研究中的应用 |
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| 引用本文： | 祝秀梅,慕洪涛,黄安仓,黄银君,卫广森.反向遗传学技术及其在口蹄疫病毒研究中的应用[J].中国畜牧兽医,2010,37(1):138-140. |
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| 作者姓名： | 祝秀梅 慕洪涛 黄安仓 黄银君 卫广森 |
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| 作者单位： | （1.中农威特生物科技股份有限公司， 兰州 730046； 2.甘肃省庆阳市动物卫生监督所， 庆阳 745000） |
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| 摘 要： | 反向遗传操作技术(reverse genetics,RG)是一种新兴的分子生物学技术,该技术可以在DNA水平上对病毒基因组进行各种修饰或改造,然后通过拯救病毒的表型变化来判断这些基因操作的效果,从而可以对病毒基因组表达调控机制、病毒致病的分子机理等进行研究。作者就口蹄疫病毒反向遗传研究的意义、方法及应用等方面进行了综述。
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| 关 键 词： | 口蹄疫病毒 反向遗传学 全长cDNA |
Reverse Genetic Technique and its Application in Foot-and-mouth Disease Virus |
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| ZHU Xiu-mei,MU Hong-tao,HUANG An-cang,HUANG Yin-jun,WEI Guang-sen.Reverse Genetic Technique and its Application in Foot-and-mouth Disease Virus[J].China Animal Husbandry & Veterinary Medicine,2010,37(1):138-140. |
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| Authors: | ZHU Xiu-mei MU Hong-tao HUANG An-cang HUANG Yin-jun WEI Guang-sen |
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| Institution: | （1.China Agricultural Veterinary Biological Science and Technology Co.Ltd，Lanzhou 730046， China;2.Qingyang Animal Health Inspection in Gansu Province, Qingyang 745000， China） |
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| Abstract: | Reverse genetics is a new developed molecular biology technique.It can run a variety of modify and transform on DNA level for virus genome,and then these genetic manipulation effect is judged by phenotype change of rescued virus.So it can be used to research the expression and regulation mechanism of virus genome and molecular mechanism of virus pathogenicity.The technique has been applied in foot-and-mouth disease virus research,the purpose,methods and applications were also reviewed. |
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| Keywords: | foot-and-mouth disease virus reverse genetic full-length cDNA |
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