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珍珠李枝枯病病原鉴定及致病性测定
引用本文:黄荣,唐利华,郭堂勋,李其利,孙文秀,黄穗萍,陈小林,莫贱友. 珍珠李枝枯病病原鉴定及致病性测定[J]. 植物病理学报, 2022, 52(5): 863-866
作者姓名:黄荣  唐利华  郭堂勋  李其利  孙文秀  黄穗萍  陈小林  莫贱友
作者单位:广西壮族自治区农业科学院植物保护研究所/广西作物病虫害生物学重点实验室,南宁 530007;
长江大学生命科学学院,荆州 434025
基金项目:广西重点研发计划(桂科AB20159041);广西作物病虫害生物学重点实验室自主研究课题(20-065-30-ST-04)
摘    要:

收稿时间:2021-09-02

Identification and pathogenicity test of the pathogens of branch blight on pearl plum
HUANG Rong,TANG Lihua,GUO Tangxun,LI Qili,SUN Wenxiu,HUANG Suiping,CHEN Xiaolin,MO Jianyou. Identification and pathogenicity test of the pathogens of branch blight on pearl plum[J]. Acta Phytopathologica Sinica, 2022, 52(5): 863-866
Authors:HUANG Rong  TANG Lihua  GUO Tangxun  LI Qili  SUN Wenxiu  HUANG Suiping  CHEN Xiaolin  MO Jianyou
Affiliation:Institute of plant protection, Guangxi Academy of Agricultural Sciences / Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning 530007, China;
Yangtze University, Jingzhou 434025, China
Abstract:In order to confirm the pathogen of branch blight on pearl plum and its virulence, 14 samples were collected from Guangxi and Guizhou Province, and 21 isolates were obtained and were identified as Botryosphaeriaceae. The results of colony morphology, conidial observation and molecular identification of internal transcribed spacer region (ITS1-5.8S-ITS2) and the translation elongation factor 1-α (EFl-α) showed that the pathogens of branch blight on pearl plum were Botryosphaeria dothidea (66.67%), Neofusicoccum parvum (19.05%), N. algeriense (9.52%), and Lasiodiplodia pseudotheobromae (4.76%). The pathogenicity of different species of Botryosphaeriaceae was tested by mycelial dishes inoculation, and it was found that typical isolates of the four species were pathogenic, among which L. pseudotheobromae was the most virulent one and B. dothidea was the weakest one. This is the first report that L. pseudotheobromae causes branch blight on pearl plum.
Keywords:pearl plum  branch blight  identification of pathogens  pathogenicity test  
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