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| 芒果蒂腐病病原葡萄座腔菌科真菌种类鉴定 | |
| 引用本文： | 孙秋玲,杨芝霓,唐利华,黄穗萍,陈小林,李其利,郭堂勋,莫贱友,韦继光,罗来凤.芒果蒂腐病病原葡萄座腔菌科真菌种类鉴定[J].植物病理学报,2022,52(6):1009-1012. |
| 作者姓名： | 孙秋玲 杨芝霓 唐利华 黄穗萍 陈小林 李其利 郭堂勋 莫贱友 韦继光 罗来凤 |
| 作者单位： | 广西大学农学院,南宁 530003; 广西农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,南宁 530005; 广西桂林市经济作物技术推广站,桂林 541002; 广西壮族自治区国有钦廉林场,钦州 535424 |
| 基金项目： | 财政部和农业农村部:国家现代农业产业技术体系资助(CARS-31);广西作物病虫害生物学重点实验室基金项目(20-065-30-ST-06) |
| 摘 要： | |
| 收稿时间： | 2021-10-21 |
Identification of pathogenic Botryosphaeriaceae species from mango stem-end rot |
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| SUN Qiuling,YANG Zhini,TANG Lihua,HUANG Suiping,CHEN Xiaolin,LI Qili,GUO Tangxun,MO Jianyou,WEI Jiguang,LUO Laifeng.Identification of pathogenic Botryosphaeriaceae species from mango stem-end rot[J].Acta Phytopathologica Sinica,2022,52(6):1009-1012. | |
| Authors: | SUN Qiuling YANG Zhini TANG Lihua HUANG Suiping CHEN Xiaolin LI Qili GUO Tangxun MO Jianyou WEI Jiguang LUO Laifeng |
| Institution: | College of Agriculture, Guangxi University, Nanning 530003, China; Institute of Plant Protection, Guangxi Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Crop Pest Biology, Nanning 530005, China; Guangxi Guilin Popularization Station of Cash Crop Technique, Guilin 541002, China; Guangxi Qinlian State-owned Forest Farm, Qinzhou 535424, China |
| Abstract: | In order to identify the pathogen of Botryosphaeriaceae causing mango stem-end rot, tissue isolation method was used to process mango samples collected from Hainan, Guangxi, Yunnan, and Sichuan Provinces in China, and 58 fungal isolates were obtained. Among them, 39 isolates were belonged to Botryosphaeriaceae, accounting for 67.24%. These isolates were identified based on morphological characteristics and phylogenetic analysis with internal transcribed spacer (ITS) and elongation factor 1 alpha (EF-1α). The results showed that there were four main species of Botryosphaeriaceae, including Neofusicoccum parvum (33.33%), Lasiodiplodia theobromae (30.77%), Botryosphaeria dothidea (28.21%), and L. pseudotheobromae (7.69%). The mango stem-end rot caused by L. pseudotheobroma was firstly reported in China. The virulence of the four species was tested by in vitro inoculation and the results showed that all isolates were pathogenic, among which L. theobromae, L. pseudotheobroma, and N. parvum were the “strong” grade, and B. dothidea was the “middle” grade. |
| Keywords: | mango stem-end rot Botryosphaeriaceae pathogenic identification |
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