乳及乳制品中牛乳铁蛋白含量测定方法比较研究

目的 探讨两种不同检测方法测定乳与乳制品中牛乳铁蛋白的含量及应用研究。方法 对比高效液相色谱（HPLC）法和超高效液相色谱串联质谱（UPLC- MS/MS）法测定生鲜乳、调制乳、超高温瞬时灭菌乳、含乳饮料和奶粉样品等乳及乳制品中牛乳铁蛋白的含量,并对测定结果进一步比较和分析原因。结果 两种测定方法均具有较高回收率和较优的重现性,但两者方法对实际样品的测定结果具有一定差异性。结论 HPLC法测定牛乳铁蛋白是通过肝素亲和柱纯化富集乳铁蛋白,测定的是非热变性牛乳铁蛋白的含量;而UPLC-MS/MS法测定的是热变性和非热变性牛乳铁蛋白的含量,不受热处理加工工艺的影响。实验室可针对不同检测目的选择不同方法进行定量研究。

Comparative Study on Determination Methods of Bovine Lactoferrin in Dairy Products

Objective] To investigate two different methods for the determination of lactoferrin in dairy products. Method] The content of lactoferrin in raw milk modified milk,UHT milk,milk beverage and milk powder were determined by High performance liquid chromatography（HPLC）and Ultra high performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS）,and further analyzed the reasons for the results. Result] The two methods had high recovery and good reproducibility. However,there were some differences between the two methods. Conclusion] The determination of lactoferrin by HPLC is to purify and enrich lactoferrin by heparin affinity column,and the content of non heat denatured lactoferrin is determined;UPLC-MS/MS method is used to determine the content of heat denatured and non heat denatured lactoferrin,which is not affected by the processing technology of heat treatment. Therefore,the laboratory can choose different methods for quantitative research for different testing purposes.