稻瘟病菌与丽江新团黑谷苗期互作叶片代谢产物分析

 为了从代谢产物角度解析稻瘟病菌诱导 ‘丽江新团黑谷’(LTH)叶片的感病机理,本研究采用GC-MS检测稻瘟病菌接种后LTH叶片和对照LTH叶片的代谢产物,利用在线软件进行处理之间代谢产物的主成分分析(Principal component analysis,PCA)、偏最小二乘判别分析(Partial least-squares discriminantion analysis, PLS-DA)和差异代谢通路分析。结果表明:GC-MS定性到LTH叶片中331种代谢物,PCA和PLS-DA分析可以将接种叶片与对照叶片的代谢产物分开,从接种后2 d开始,随着接种时间延长接种叶片与对照叶片的代谢产物相距越来越远。稻瘟病菌诱导LTH叶片中1-单油酸、胆固醇、葡萄糖酸、别嘌呤醇、黄嘌呤、肌苷和菊粉糖相对含量显著上调(VIP>1.0);15条代谢途径受到影响,其中赖氨酸降解、苯丙氨酸代谢和甜菜素生物合成的-lnP值随着接种时间延长逐渐增大。推测,稻瘟病菌侵染导致LTH叶片中胆固醇等含量显著上调,赖氨酸降解、苯丙氨酸代谢和甜菜素生物合成代谢受到干扰,进而促使叶片组织坏死。

The metabolic response of rice seedling of Lijiangxintuanheigu variety after Magnaporthe oryzae inoculation

To understand the susceptibility of the rice variety Lijiangxintuanheigu (LTH) towards the pathogen Magnaporthe oryzae, the metabolites of M. oryzae inoculated leaves and control leaves were analyzed by GC-MS. Principal component analysis (PCA), partial least-squares discriminant analysis (PLS-DA) and differential metabolic pathway analysis were performed. As a result, a total of 331 metabolites was detected by GC-MS analysis. The metabolites of the inoculated leaves could be distinguished from the control leaves by PCA and PLS-DA analysis. In M. oryzae inoculated leaves, 1-monoolein, cholesterol, gluconic acid, allopurinol, xanthine, inosine and inulotriose were significantly upregulated (VIP>1.0) comparing to the control group; meanwhile, 15 metabolic pathways were affected, among which the -lnP value of lysine degradation, phenylalanine metabolism and betalain biosynthesis was found to be gradually increased after inoculation. These results suggested that M. oryzae infection could disturb the lysine degradation, phenylalanine metabolism and betalain biosynthesis in LTH, and then promote the necrosis of leaf tissues.