椰子ALDH基因家族的鉴定及生物信息学分析

为了鉴定椰子的ALDH基因家族，分析其基因结构、保守结构域、系统发育树以及其表达模式，通过下载OsALDH基因家族的蛋白质序列，并与椰子的蛋白质的数据库进行比对，鉴定出12个CnALDH基因，分属于9个亚族。结果表明，ALDH编码的氨基酸等电点为5.35～8.68，多数分布在叶绿体中。表达谱分析结果显示，除CnALDH12＿A1和CnALDH22＿A1外，其他的CnALDH基因家族成员均在叶片中有高水平表达。而CnALDH10＿A8在不同组织以及不同时期展现组成性表达，推测该基因参与椰子的一些基础性功能，是不可或缺的基因。

Identification and bioinformatics analysis of ALDH gene family in coconut

Aldehyde dehydrogenase (ALDH), which dehydrogenates aldehydes into carboxylic acids, plays an important role in plant stress, development and fragrance synthesis. The protein sequences of OsALDH gene were downloaded and compared with the protein database of coconut, and 12 CnALDH genes were identified, which belonged to 9 subfamilies. The results showed that the isoelectric points of amino acids encoded by ALDH ranged from 5.35 to 8.68, and most of them were distributed in chloroplasts. Expression profile analysis showed that all ALDH gene family members were highly expressed in coconut young leaves except CnALDH12_A1 and CnALDH22_A1. CnALDH10_A8 showed constitutive expression in different tissues and at different periods, suggesting that CnALDH10_A8 is an indispensable gene involved in some basic functions in coconut.