基于转录组数据的生姜ZoWRKY基因家族鉴定及逆境响应分析

WRKY是一类植物特有的转录因子家族,每个成员都含有高度保守的WRKY结构域,广泛参与植物的生长发育、信号传导和逆境胁迫应答等过程。本研究基于生姜转录组数据鉴定了ZoWRKY家族成员。结果显示,在340.2Mb的生姜转录组数据中,共获得78个具有ORF序列的ZoWRKY家族成员,分为Ⅰ、Ⅱ和Ⅲ 3个亚族,其中亚族Ⅱ又分为Ⅱ-a、Ⅱ-b、Ⅱ-c、Ⅱ-d和Ⅱ-e。逆境响应表达分析显示,分布在Ⅱ-c、Ⅱ-d和Ⅲ等的21个ZoWRKY响应土壤湿度,而分布在Ⅰ、Ⅱ-a和Ⅱ-c等的19个ZoWRKY响应青枯菌侵染,推测ZoWRKY在生姜逆境响应中具有重要功能。对后续开展生姜WRKY的研究、促进抗性育种和提高产量具有重要意义。

Identification and Expression Analysis of the ZoWRKY Family in Stress Responses Based on Transcriptome Data of Ginger (Zingiber officinale Roscoe)

The WRKY proteins was a group of plant-specific transcription factors that shared WRKY conserved domains. They perform crucial roles in growth, development, signal transduction and stress response. Based on RNA-Seq data from ginger, researchers were able to identify and analyse members of the ZoWRKY family (Zingiber officinale Roscoe). The results showed that a toal of 78 putative ZoWRKY family members with complete ORF sequences were identified in 340.2Mb total nucleotides. These candidate genes could be clustered into three subfamilies:Ⅰ, Ⅱ and Ⅲ, respectively, and subfamilyⅡcould be further divided into Ⅱ-a,Ⅱ-b,Ⅱ-c,Ⅱ-d and Ⅱ-e group. The expression patterns of ZoWRKY genes showed that 21 members significantly responded to moisture stress, which were mainly distributed in Ⅱ-c,Ⅱ-d, and Ⅲ. After infected by Ralstonia solanacearum, 19 of ZoWRKY genes were differentially expressed, and were mainly distributed inⅠ,Ⅱ-a, and Ⅱ-c, which speculated that ZoWRKY has important function in stress responses. These results provide valuable information for further research of WRKY transcription factors in ginger, and can promote the resistance breeding and the yield increase of ginger.