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陕西省鸡胚源性大肠杆菌的分离鉴定
引用本文:张国祥,王晶钰,窦永喜,张彦明,刘业兵. 陕西省鸡胚源性大肠杆菌的分离鉴定[J]. 动物医学进展, 1998, 0(3)
作者姓名:张国祥  王晶钰  窦永喜  张彦明  刘业兵
作者单位:西北农业大学动物科学与动物医学学院 中国农业科学院兰州兽医研究所
摘    要:从陕西关中六个种鸡场的198只死胚及出壳弱雏中分离出98株大肠杆菌,平均分离率为49.5%。通过小鸡致病力试验,筛选出63株具有致病力的菌株,占分离菌的64.3%。生化鉴定表明,这些致病菌均符合大肠埃希氏菌的生化特性。血清型鉴定可确定6种,其中以O1、O2、O78为主,分别占鉴定菌的44.4%(28/63)、33.3%(21/63)和9.5%(6/63),另三种为O22、O75、O89共占8.0%(5/63)。有3株未定型,占4.8%(3/63)。用16种抗生素进行药敏试验,对先锋霉素V和恩诺沙星高度敏感,对羧苄青霉素、氨苄青霉素、氯霉素、卡那霉素、磺胺嘧啶、新霉素、链霉素中度敏感。

关 键 词:大肠杆菌  死亡鸡胚  弱雏  致病性  分离鉴定

Isolation and Identification of Avian Escherichia Coli from Embryo or Chicks in Shaanxi
Zhang Guoxiang wang Jingyu Dou Yongxi Zhang Yanming Liu Yebin. Isolation and Identification of Avian Escherichia Coli from Embryo or Chicks in Shaanxi[J]. Progress In Veterinary Medicine, 1998, 0(3)
Authors:Zhang Guoxiang wang Jingyu Dou Yongxi Zhang Yanming Liu Yebin
Abstract:strains of Escherichia coli were recovered from 198 dead chicken embryoes in late incubation and day old weak chicks from breeder chicken farms in Shaanxi Province.The average isolation rate of E.coli in dead embryoes and day old weak chicks were 49.5% of the all E.coli,63 strains had high pathogenicity for young chickens, serotyping identification results demonstrated that O 1,O 2 and O 78 serogroups were predominant,and the others were O 22 ,O 75 ,O 89 ;3 strains could not be serotyped.Their properties based on culture,morphology,biochemistry,pathogenicity,antibiotics sensitivity were described.Inactivated vaccine and immunological control against E.coli were studied as well.
Keywords:E.coli   dead chicken embryoes   dead in shell chicken embryoes   isolation and identification   pathogenicity  
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