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Applications of various stereological tools for estimation of biological tissues
Authors:Aymen A Warille  Adem Kocaman  Abdalla A Elamin  Hamza Mohamed  Abubaker El Elhaj  Berrin Zuhal Altunkaynak
Institution:1. Department of Anatomy, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey;2. Department of Histology and Embryology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey;3. Department of Anatomy, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah, United Arab Emirates;4. Anatomy Department, College of Medicine, University of Ha'il, Hail, Saudi Arabia;5. Department of Histology and Embryology, Faculty of Medicine, Okan University, İstanbul, Turkey
Abstract:To provide concise and brief important stereological application methods and techniques for estimating biological tissues. Stereology studies the quantity of biological tissue using little practice and the low price of counting and preparing tissue slices to obtain direct and accurate results. Since their establishment, the stereological techniques underwent much improvement, thus allowing more precise analysis of target structures using various approaches. Using stereological tools, advances in stereological techniques made the target tissues or organs represented by 2D instead of 3D dimensions. Process tools estimate volume, area and length. According to the exciting tissue and aims, the stereological tools perform differently. This review summarizes various stereological tools and techniques, providing brief information about the orientation method, slicer probe method, Delesse's principle, Cavalieri principle, disector, fractionator, nucleator, virtual cycloids and saucer, which are described in detail.
Keywords:3D  Cavalieri principle  disector  estimation  saucer  stereological techniques
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