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Biochemical Testing Method for Determining Efficiency of Chemical Treatment on Quadraspidiotus perniciosus Comst.
Authors:V.A. Kolybine
Affiliation:Institute of Zoology, Academy of Sciences, Kiev (Soviet Union)
Abstract:Efficiency of chemical control of Quadraspidiotus perniciosus Comst. can be assessed, 3–5 days after treatment, by the use of a colour reaction relating to oxyreductase activity. The optimal conditions for this reaction are a 0.5-1.5% solution of 2,3,5-triphenyl-tetrazolium chloride (pH 10.5) and time of 2–3 h. It is noted that use of a biochemical test for assessing mortality is possible for other insects, and is not dependent on the reasons causing their death.
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