Identification of bovine serum albumin as an IgE-reactive beef component in a dog with food hypersensitivity against beef |
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Authors: | Ohmori Keitaro Masuda Kenichi Kawarai Shinpei Yasuda Nobutaka Sakaguchi Masahiro Tsujimoto Hajime |
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Affiliation: | Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. |
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Abstract: | IgE-reactive beef components were examined by an immunoblot analysis using a serum from a dog with food hypersensitivity against beef. The immunoblot analysis revealed a distinct band at approximately 66 kDa and a faint band at approximately 50 kDa. The immunoblot analysis for serum IgE reactivity to bovine serum albumin (BSA) also revealed a positive band at 66 kDa. Serum IgE reactivity to the 66-kDa protein of beef was diminished by pre-incubating the serum sample with BSA. Furthermore, a positive reaction to BSA was detected in intradermal testing in the dog. These results clearly indicated that BSA was an IgE-reactive beef component in the dog with food hypersensitivity against beef. |
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