| 中国林蛙与黑龙江林蛙血清蛋白比较分析 |
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| 引用本文： | 朴忠万,金建丽,金志民,杨春文,闫兵.中国林蛙与黑龙江林蛙血清蛋白比较分析[J].安徽农业科学,2009,37(25):12039-12040. |
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| 作者姓名： | 朴忠万 金建丽 金志民 杨春文 闫兵 |
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| 作者单位： | 牡丹江师范学院生物系,黑龙江牡丹江,157012 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 目的]为林蛙种属的科学划分提供依据。方法]以中国林娃和黑龙江林娃为材料，采用聚丙烯酰胺凝胶电泳法对其血清蛋白进行比较分析。结果]从中国林蛙血清中共分离出25条蛋白带，从黑龙江林娃血清中共分离出23条蛋白带，其中迁移率为0．552、0．524、0．400、0．375、0．331、0．241、0．076、0．062、0．048的9条蛋白带为中国林蛙特有；迁移率为0．517、0．269、0．228、0．214、0．127、0．069、0．041的7条蛋白带为黑龙江林娃特有，迁移率为0．621、0．469、0．434、0．359、0．317、0．297、0．200、0．172、0．159、0．145、0．124、0．103、0．090、0．055、0．028、0．014的16条蛋白带为两者共有。结论]中国林娃与黑龙江林娃应归于2个独立的蛙种。
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| 关 键 词： | 中国林娃 黑龙江林娃 血清蛋白 迁移率 |
Comparative Analysis on Serum Protein of Rana chensinensis and Rana amurensis |
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| PIAO Zhong-wan et al.Comparative Analysis on Serum Protein of Rana chensinensis and Rana amurensis[J].Journal of Anhui Agricultural Sciences,2009,37(25):12039-12040. |
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| Authors: | PIAO Zhong-wan |
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| Institution: | PIAO Zhong-wan et al (Biological Department of Mudanjiang Normal College,Mudanjiang,Heilongjiang 157012) |
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| Abstract: | Objective] The study was to provide the basis for the scientific classification of rana species.Method] With Rana chensinensis and Rana amurensis as the materials,their serum protein was comparatively analyzed by polyacrylamide gel electrophoresis method.Result] 25 protein bands were separated from R.chensinensis serum and 23 protein bands were separated from R.amurensis serum.9 protein bands which mobility were 0.552,0.524,0.400,0.375,0.331,0.241,0.076,0.062,0.048 resp.were the particular bands of R.che... |
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| Keywords: | Rana chensinensis Rana amurensis Serum protein Mobility |
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