青海盐湖地区嗜盐菌的分离纯化及抑制植物病原菌的活性初探

以番茄灰霉菌(Botrytis cinerea)、菊芋菌核菌(Jerusalem artichoke Sclerotium)、油菜菌核菌(Sclerotinia sclerotiorum)、辣椒疫霉菌(Phytophthora capsici)、蚕豆根腐菌(Fusarium solani)及豌豆根腐菌(Aphaomyces euteiches Dreehsler)等6种植物病原真菌为供试菌株,采用滤纸片法对植物病原菌提取液的抑菌活性进行了测定.为了获得对这几种植物病原菌具有较高抑制作用的嗜盐菌菌株,对供试菌株进行了液体发酵及发酵液的活性筛选.经滤纸片法测定,青海盐湖嗜盐菌菌株对辣椒疫霉菌具有较强的拮抗作用,并用最小抑制浓度法(MIC)测定了嗜盐菌提取物对辣椒疫霉菌的最小抑菌浓度范围为9～12 mg/mL.

Isolation, petrifaction and inhibitory activity against plant pathogenic fungi of halophilic strains from Qinghai Salt Lake

Six plant pathogenic fungi including Botrytis cinerea, Jerusalem artichoke Sclerotium, Sclerotinia sclerotiorum, Phytophthora capsici, Fusarium solani, and Aphanomyces euteiches Dreehsler were used to determine the inhibitory activity of fermentation broth of halophilic bacteria against plant pathogenic fungi by paper disc diffusion method. In order to find halophilic strain with higher inhibitory activity against the plant pathogenic fungi, the tested strains were fermented to screen. The halophilic strains were with certain antagonistic activity against the growth of P.capsici by the disc diffusion assay. And the minimum inhibitory concentration (MIC) of fermentation broth of halophilic strains against P.capsici ranged from 9 to 12 mg/mL.